華中農(nóng)業(yè)大學(xué)植物科技學(xué)院導(dǎo)師:金雙俠

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華中農(nóng)業(yè)大學(xué)植物科技學(xué)院導(dǎo)師:金雙俠

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華中農(nóng)業(yè)大學(xué)植物科技學(xué)院導(dǎo)師:金雙俠 正文


  基本信息
  姓名: 金雙俠 出生年月: 1979.2
  性別: 男 碩/博導(dǎo): 碩導(dǎo)
  民族: 漢 開設(shè)課程: 植物生物技術(shù),作物生產(chǎn)實習(xí),育種學(xué)實習(xí)
  職稱: 副教授 研究方向: 棉花組織培養(yǎng),基因工程
  學(xué)位: 博士

  聯(lián)系方式
  辦公電話:027-87283955
  電子郵件:

  個人簡介
  華中農(nóng)業(yè)大學(xué)植物科學(xué)技術(shù)學(xué)院作物遺傳育種專業(yè)博士,副教授,碩士生導(dǎo)師。1997-2001,華中農(nóng)業(yè)大學(xué)農(nóng)學(xué)系農(nóng)學(xué)專業(yè)就讀;2001-2006,華中農(nóng)業(yè)大學(xué)植物科學(xué)技術(shù)學(xué)院攻讀碩士、博士學(xué)位,獲農(nóng)學(xué)博士學(xué)位,指導(dǎo)教師均為張獻(xiàn)龍教授. 目前在美國University of Central Florida, Henry Daniell 教授指導(dǎo)下進(jìn)行博士后研究,研究方向為植物葉綠體轉(zhuǎn)化。研究領(lǐng)域主要涉及棉花細(xì)胞、組織培養(yǎng)、棉花轉(zhuǎn)基因等。目前從事棉花抗病,蟲及逆境基因的克隆及高效表達(dá)載體(葉綠體轉(zhuǎn)化表達(dá)體系)的開發(fā)及轉(zhuǎn)基因研究。
        
  科研項目
  國家自然科學(xué)基金 植物凝集素基因在棉花中的功能分析 主持 2012-2015
  新疆建設(shè)兵團博士資金項目 轉(zhuǎn)基因抗病蟲、抗逆聚合雜交棉新組合選育技術(shù)研究,主持,2007-2009
  國家自然科學(xué)基金 棉花外源基因葉綠體轉(zhuǎn)化體系的建立及其應(yīng)用(30771368),主持,2008-2010
  教育部博士點新教師基金 以gfp基因為報告基因監(jiān)測轉(zhuǎn)基因棉花花粉漂移(20070504087),主持,2008-2010
  國家自然科學(xué)基金重大國際合作項目 Development of strong pest-resistance cotton via transforming plastid and its application in breeding, 主要參加者,2009-2011
  國家自然科學(xué)基金 利用基因誘捕構(gòu)建棉花功能基因組研究突變?nèi)后w,主要參加,2005-2007
   
  發(fā)明專利及獲獎情況
  獲獎情況:
  湖北省優(yōu)秀博士學(xué)位論文(第10批)獲獎?wù)撸?008年
  湖北省優(yōu)秀本科論文指導(dǎo)獎,2008年。本人指導(dǎo)的2007界本科生張碧瑤,其論文:新疆棉花高體細(xì)胞胚胎發(fā)生能力基因型的篩選,獲得湖北省優(yōu)秀本科學(xué)位論文。
  獲第十、十一屆湖北省自然科學(xué)優(yōu)秀論文一等獎2次
  申報兩項發(fā)明專利:
  超聲波輔助農(nóng)桿菌轉(zhuǎn)化棉花胚芽的方法,專利申請?zhí)枺?00710063650.0
  一種分離棉花葉綠體DNA的方法,專利申請?zhí)枺?00710052665.7
    
  發(fā)表的論文及著作
   [1]Shuangxia Jin, Xianlong Zhang1, Henry Daniell* Pinellia ternata agglutinin expression in chloroplasts confers broad spectrum resistance against aphid, whitefly, lepidopteran insects, bacterial and viral pathogens.Plant Biotechnology Journal,2011,online.(SCI)
   [2]Dheeraj Verma, Shuangxia Jin, Anderson Kanagaraj, Nameirakpam Dolendro Singh, Jaiyanth Daniel, Pappachan Kolattukudy, Michael Miller, and Henry Daniell.Membrane Destabilization Properties of Cutinase and Swollenin expressed in Transgenic Chloroplasts.(Under review)
   [3]Shuangxia Jin, Anderson Kanagaraj, Dheeraj Verma, Theo Lange, Henry Daniell.Release of Hormones from Conjugates: Chloroplast Expression of ß-glucosidase Results in Elevated Phytohormone Levels Associated with Significant Increase in Biomass and Protection from Aphids or Whiteflies Conferred by Sucrose Esters. Plant Physiol,2011,155,222-235.(SCI)
   [4]Seung-Bum Lee, Baichuan Li, Shuangxia Jin and Henry Daniell.Expression and characterization of antimicrobial peptides Retrocyclin-101 and Protegrin-1 in chloroplasts to prevent sexual transmission of diseases. Plant Biotechnology Journal.2011,9.100-115.(SCI)
   [5]Verma D, Kanagaraj A, Jin SX, Singh N, Kolattukudy P, Daniell H. Chloroplast-derived enzyme cocktails hydrolyse lignocellulosic biomass and release fermentable sugars.Plant Biotechnology Journal,2010,8:332-350 (SCI).
   [6]Jin SX, Zhang XL, Zhu HG, Zhou ZL, Nie YC. Detection of somaclonal variation of cotton (Gossypium hirsutum) through RAPD, SSR markers, cytogenetic and flow cytometer analysis. Plant Cell Reports, 2008,27:1303–1316.(SCI)
   [7]Jin SX, Zhang XL, Guo XP, Nie YC. Transformaton of Upland cotton with gfp gene as a visual marker. Biologia Plantarum, Accepted(SCI).
   [8]Shuangxia Jin, Xianlong Zhang, et al, Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton. Biologia Plantarum, 2006 50(4):519-524 .(SCI)
   [9]Shuangxia Jin, Xianlong Zhang(2006) An efficient grafting system for transgenic plant recovery in cotton (Gossypium hirsutum L.). Plant Cell, Tissue and Organ Culture, 85(5): 181-185 .(SCI)
   [10]Shuangxia Jin, Xianlong Zhang (2005) Factors affecting stable transformation and plant regeneration during transforming embryogenic callus of Upland cotton (Gossypium hirsutum L.) via Agrobacterium tumefaciens, Plant Cell, Tissue and Organ Culture, 81(2): 229-237 .(SCI)
   [11]Shuangxia Jin, Xianlong Zhang (2005) Function Analysis of PromoterTrapping System after Inserting Cotton (Gossypium hirsutum L.) Genome. J GENET GENOMICS (Acta Genetica Sinca), 32(12):1266-1274.
   [12] Xianlong Zhang and Shuangxia Jin (2007) Transgenic Cotton: An Overview(invited review). Transgenic Plant Journal, 2007, 1(1), 150-162.
   [13] Fang Ding, Shuangxia Jin, Ni Hong, Yun Zhong, Qing Cao, Ganjun Yi and Guoping Wang. 2008. Vitri?cation–cryopreservation, an ef?cient method for eliminating Candidatus Liberobacter asiaticus, the citrus Huanglongbing pathogen, from in vitro adult shoot tips. Plant Cell Reports,27(2),241-250 (SCI)
   [14] Sun Y Q, Zhang X L, Jin S X. Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion. Theor Appl Genet, 2004, 109: 472-479 (SCI)
   [15] Sun Y Q, Zhang X L, Jin S X Somatic embryogenesis and plant regeneration in wild cotton (Gossypium klotzschianum Anderss). Plant Cell Tissure Organ Cult, 2003, 75: 247-253 (SCI)
   [16] Wu J H, Zhang X L, Jin S X.Factors affecting somatic embryogenesis and plant regeneration from a range of recalcitrant genetypes of Chinese cotton.In vitro Cell Dev Biol-Plant, 2004, 40: 371-375 (SCI)
   [17] GUO X, JIN S, ZHANG X(2007)Agrobacterium-mediated transformation of Cry1C, Cry2A and Cry9C genes into Gossypium hirsutum and plant regeneration. Biologia Plantarum, 51(2): 242-248 (SCI)
   [18] Xiyan Yang, Xianlong Zhang, Shuangxia Jin, Lili Fu, Linggang Wang Production and characterization of asymmetric hybrids between upland cotton Coker201 (Gossypium hirsutum) and wild cotton (Gossypium klozschianum Anderss),Plant Cell Tissure Organ Cult, 2007, 89:225–235 (SCI)
   [19] Yang X, Guo X, Zhang X, Nie Y and Jin S. Plant regeneration from Gossypium davidsonii protoplasts via somatic embryogenesis. BIOLOGIA PLANTARUM, 51 (3): 533-537, 2007 (SCI)
   [20] Xianlong Zhang,Shuangxia Jin, Diqiu Liu, Zhongxu Lin, Fanchang Zeng, Longfu Zhu, Lili Tu, Xiaoping Guo. Cotton Biotechnology: Challenge the Future for Cotton Improvement, In: Biotechnology in Crop Improvement, GP Rao (ed.), Studium Press, LLC, Houstan, Texas, USA. Pp. 241-301
   [21] Lili Tu, Xianlong Zhang, Diqiu Liu, Shuangxia Jin, Jinglin Cao, Longfu Zhu, Fenglin Deng, Jiafu Tan, Cunbin Zhang, 2007, Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis, Chinese Science Bulletin 52: 3110-3117 (SCI)
   [22] 周小鳳,金雙俠,李保成,余渝,寧新柱,王旭文 .初步研究不同激素對海島棉體細(xì)胞胚胎發(fā)生的影響.中國農(nóng)學(xué)通報2009,25(11):78-83
   [23]朱華國, 涂禮莉, 金雙俠, 徐理, 譚家福, 鄧鋒林, 張獻(xiàn)龍.棉花細(xì)胞初始脫分化的基因差異表達(dá)分析.科學(xué)通報, 2008,53 (20),2483-492 (SCI)
   [24] 曹景林 張獻(xiàn)龍 金雙俠 楊細(xì)燕 朱華國 付莉莉.棉花高效體細(xì)胞胚發(fā)生及同步控制培養(yǎng)體系研究.作物學(xué)報,2008, 34(2): 224−231
   [25]謝德意,金雙俠,郭小平,張獻(xiàn)龍.長江和黃河流域棉區(qū)棉花品種體細(xì)胞胚胎發(fā)生和植株再生能力研究,作物學(xué)報,2007,33(3):394-400
   [26] 謝德意,金雙俠,郭小平,張獻(xiàn)龍.棉花ipt基因遺傳轉(zhuǎn)化效率及優(yōu)化成苗技術(shù)研究,作物學(xué)報,2007,33(5)
   [27] 金雙俠, 韓杰,劉小云,劉冠澤,王一嫻,唐文鑫,張獻(xiàn)龍.SDS-蛋白酶法分離棉花cpDNA及psbA基因啟動子、終止子克隆.分子植物育種,2007,5, 683-689.
   [28] 周小鳳,張碧瑤,劉冠澤,高巍,余渝,鄧福軍,李保成,孔憲輝,張獻(xiàn)龍, 金雙俠*.新疆棉花高體細(xì)胞胚胎發(fā)生能力基因型的篩選,分子植物育種, 2007,6, 1-8(通訊作者).
   [28] 張獻(xiàn)龍,孫玉強,吳家和,金雙俠,聶以春,郭小平,2004,棉花細(xì)胞工程及新種質(zhì)創(chuàng)造,棉花學(xué)報,16(6):368-373
  
   

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